WT: 5' CACGTGTTCTAACC 3'
MUTANT: 5' CACGTGTTTTAACC 3'
The sequence of the original DNA was the compliment of these sequences, or:
WT: 5' GTGCACAAGATTGG 3'
MUTANT: 5' GTGCACAAAATTGG
3'
b) By comparing the sequences you can see that a G (in red) in
the wild type has been repaced with an A (in red) in the Mutant.
Note you can also deduce what the mutation was directly from
the difference in patterns of bands on the gel ( shown below in red below).
WT
MUTANT
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G A
T C
G A
T C
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