Solution to PS 8 #11
Although the recognition sites differ for BglII and BmH1, the enzymes leave identical sticky ends (shown below in red). The fragments can be ligated as easily as fragments produced by a single enzyme.
Bgl
II -A
GATC
T-
-T
CTAG
A-
Bam
HI -G
GATC
C-
-C
CTAG
G-
Return to Problem Set #8