Problem Set #2: Amino Acids and Peptides

1) Given the following peptide: Val-Met-Ser-Ile-Phe-Arg-Cys-Tyr-Leu

2) Draw the following amino acids in their fully protonated forms: 3) For each of the amino acids in problem #2, indicate which protons are dissociable.

4) Using the pKa' values given in the attached table, for each of the amino acids listed in problem #2, indicate the ionic form which predominates at :

5) Draw a titration curve for each of the amino acids given in problem #2.

6) Based on the information given in the attached table, is there any amino acid that could serve as a buffer at pH 8.0? If so which? Calculate the ratio of the two forms of this amino acid that would be present at this pH.

7) Draw the structure for each of the following peptides in their fully protonated forms. Indicate which protons are dissociable in the structure.

8) Draw a titration curve for each of the peptides given in Problem #7.

9) For the peptides given in Problem #7, predict the ionic form that will predominate at

10) Using the pKa's given, calculate the isoelectric point for each of the following: 11) The sequence of a  29 aa long peptide can be determined from the following data.
 
a)  Treatment of the peptide with dansyl chloride reveals that the  amino terminal  is Val.

b) Trypsin digestion, separation of peptides and Edman degradation give sequences for peptide fragments as follows:

Val-Gly-Ala-His-Ala-Gly-Glu-Tyr-Gly-Ala-Glu-Ala-Thr-Glu
Ala-Ala-Trp-Gly-Lys
Val-Leu-Ser-Pro-Ala-Lys
Thr-Asn-Val-Lys

c) Chymotrypsin digestion, separation of peptides, and Edman degradation give the following sequences for fragments:

Gly-Ala-Glu-Ala-Thr-Glu
Gly-Lys-Val-Gly-Ala-His-Ala-Gly-Glu-Tyr
Val-Leu-Ser-Pro-Ala-Lys-Thr-Asn-Val-Lys-Ala-Ala-Trp


What is the sequence for the  peptide?

12)  Predict the fragments that will be generated from the treatment of the following peptide with: a) trypsin; b) chymotrypsin; c) and S. aureus V8 protease:

Gly-Ala-Trp-Arg-Asp-Ala-Lys-Glu-Phe-Gly-Gln



Solutions to Problem Set 2:
 
Solution to #1

Solution to #2

Solution to #3

Solution to #4

Solution to #5

Solution to #6

Solution to #7

Solution to #8

Solution to #9

Solution to #10

Solution to #11

Solution to #12