amino acid + tRNA + ATP -> AMP + aminoacyl-tRNA + PPi
PPi + H2O -> 2Pi
1 GTP is hydrolyzed in each of the following steps:
Initiation (making the 70S initiation complex)
Insertion of the aminacyl-tRNA in the A site (during elongation)
Translocation (during elongation)
Termination of translation
So for a 600 aa peptide to be synthesized the total number of phosphoanhydride bonds hydrolyzed is 2400 since:
Activation:
1200 (2 x 600)
Initiation:
1
Insertion:
599
Translocaton:
599
Termination:
1
Total:
2400